Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola.
نویسندگان
چکیده
A 53-kDa protein from the outer sheath of the oral spirochete Treponema denticola was purified to homogeneity and shown to reconstitute channels in black lipid bilayer model membranes. The channel had a single-channel conductance of 1.8 nS in 0.1 M KCl, making this the largest porin channel observed to date (estimated diameter, 3.4 nm). Electron micrographs of 53-kDa-protein-containing outer sheaths of T. denticola showed a regular hexagonal array of darker staining pits.
منابع مشابه
A major antigen on the outer envelope of a human oral spirochete, Treponema denticola.
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عنوان ژورنال:
- Infection and immunity
دوره 61 5 شماره
صفحات -
تاریخ انتشار 1993